Part:BBa_K346038:Design

PmerT promoter+T3 polymerase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2650
  

Design Notes

We cut the divergent PmerT promoter to a single direction one so that it can only regulate its downstream gene's expression.

Source

T3 polymerase is from T3 phage. PmerT promoter is from Tn21.